|
|
|
| Registros recuperados: 34 | |
|
|
| Bezuidenhout, C.M.; Denman, S.; Kirk, S.A.; Botha, W.J.; Mostert, L.; McLeod, A.. |
| Agathosma species, which are indigenous to South Africa, are also cultivated for commercial use. Recently growers experienced severe plant loss, and symptoms shown by affected plants suggested that a soilborne disease could be the cause of death. A number of Phytophthora taxa were isolated from diseased plants, and this paper reports their identity, mating type, and pathogenicity to young Agathosma plants. Using morphological and sequence data seven Phytophthora taxa were identified: the A1 mating type of P. cinnamomi var. cinnamomi, P. cinnamomi var. parvispora and P. cryptogea, the A2 mating type of P. drechsleri and P. nicotianae, and two homothallic taxa from the P. citricola complex. The identity of isolates in the P. citricola complex was resolved... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Avocado; Buchu; Fynbos; Glucose-6-phosphate isomerase; Isozymes; Malate dehydrogenase; Pathogenicity; Root-rot; Taxonomy. |
| Ano: 2010 |
URL: http://www.repository.naturalis.nl/record/532083 |
| |
|
|
| Cabral,Betânia Lúcia Rocha; Souza,Joelson Araújo de; Ando,Akihiko; Veasey,Elizabeth Ann; Cardoso,Eloisa Maria Ramos. |
| Cassava (Manihot esculenta Crantz) belongs to the Euphorbiaceae family, and is widely cultivated in Brazil. The objective of this study was to evaluate the isoenzymatic variability of 200 cassava accessions from the germplasm bank of Embrapa Amazonia Oriental. Seven groups were formed according to their origin: 1-Amazonas, 2-Amapa, 3-Bahia, 4-Para, 5-Rondonia, 6-Various, for accessions with a maximum of three individuals per place of origin, and 7 - Accessions of indefinite origin. The accessions were also evaluated as a whole. For the electrophoretic analyses, samples of young leaves were used in a 12% starch gel. Eight isoenzymatic systems were evaluated: acid phosphatase (ACP), leucine aminopeptidase (LAP), glucose-6-phosphate dehydrogenase (G6PDH),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Genetic variability; Germplasm bank; Isozymes. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000300017 |
| |
|
|
| Chacoff,N P; Souto,C P; Aizen,M.A.; Premoli,A. |
| Increasing crop genetic diversity has the potential to enhance pollination services, by contributing to the long-term stability of agroecosystems. Different species of the genus Citrus, including grapefruit, are cultivated worldwide. In Argentina, 30% of the total national yield is concentrated in the northwestern part of the country where new cultivars have arisen. We analyzed genetic variation and diversity within and among four plantations of Citrus paradisi for 11 isozyme loci. Although representing single cultivars clonally propagated, within plantations genetic variation was high. Observed heterozygosity ranged from 33 to 36.5%. The number of alleles per locus ranged from 2.4 to 2.6, and polymorphism from 72.7 to 81.8% and for the species in the area... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Isozymes; Genetic variation; Upper Bermejo River Basin. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332009000200001 |
| |
|
|
| Cinto,Isabel E.; Dokmetzian,Diana A.; Ranalli,María E.. |
| The aim of this study was to delimit two Iodophanus species: I. carneus and I. testaceus , based on morphological characteristics and electrophoretic patterns of their intracellular isozymes. Twenty monosporic strains were used, including five belonging to I. granulipolaris as a control. Fourteen isozyme systems were tested, and the five having the best resolution selected: aspartate aminotransferase, esterases, alkaline phosphatase, glutamate dehydrogenase, and superoxide dismutase. These analyses confirmed the similarity between I. carneus and I. testaceus , since they both produced the same band patterns, which were in turn different from the band pattern of I. granulipolaris. So, as we couldn´t find any character wich permit us to classify the isolated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungi; Lodophanus; Isozymes; Synonymy; Taxonomy. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000200005 |
| |
|
|
| Collares,Eliana Antonia Silveira; Choer,Eva; Pereira,Arione da Silva. |
| The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; Electrophoresis; Isozymes; RAPD; Genetic similarity. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000900006 |
| |
|
| |
|
|
| Deng,JB; Ding,CB; Zhang,L; Zhou,YH; Yang,RW. |
| Four isozymes, superoxide dismutase (SOD), polyphenol oxidase (PPO), malate dehydrogenase (MDH) and cytochrome oxidase (COD) were studied for identification of six herbal species (Curcuma L.). All the 37 study specimens produced a total of 168 polymorphism isozyme bands. The genetic distance coefficients (GS) varied from 0.08 to 0.54. The dendrogram, obtained according to the polymorphism isozyme bands by the UPGMA method with the software NTSYS-pc2.1, contributed to improve the resolution of phylogeny. From the dendrogram, it was possible to differentiate between the wild and cultivated specimens of C. longa, and within C. sichuanensis species. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Curcuma; Isozymes; Genetic relationship; Phylogeny. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572011000200010 |
| |
|
|
| ENÉAS-FILHO,JOAQUIM; BARBOSA,GISLAINY KARLA DA COSTA; SUDÉRIO,FABRÍCIO BONFIM; PRISCO,JOSÉ TARQUÍNIO; GOMES-FILHO,ENÉAS. |
| Three isoforms of beta-galactosidases were isolated and partially purified from the cotyledons of quiescent seeds of Vita 3 and Vita 5 cowpea [Vigna unguiculata (L.) Walp.] cultivars differing in water and salt stress tolerance. The purification procedure consisted of ammonium sulfate fractionation, acid precipitation, ion exchange chromatography through DEAE-sephadex and affinity chromatography through Lactosyl-sepharose columns. The three isoforms isolated from the two cultivars showed the same chromatographic patterns, same optimum of temperature for enzyme activity assay (60ºC), identical thermal stability up to 50°C, and similar pH optima (3-4). However, they differed from each other in sensitivity towards metal ions and certain chemical agents... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cotyledons; Isozymes; Vigna unguiculata; Vita 3 and Vita 5 cultivars; Salt tolerance. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300001 |
| |
|
| |
|
|
| González González, Miguel. |
| Para diseñar estrategias de conservación in situ de la diversidad genética del maíz, se requiere de valorar tanto a la diversidad como al potencial para incrementar la producción bajo el esquema de la agricultura tradicional como punto de referencia. Dado que el intercambio de semilla se da principalmente dentro de las comunidades rurales, se consideró al municipio de Ayapango, Edo. de Méx., para colectar las poblaciones de maíz que se manejan en aproximadamente el 20% de los hogares. Se colectaron 97 poblaciones de la raza Chalqueño, y dos de Cacahuacintle. Dentro de Chalqueño hubo poblaciones del tipo de grano cremoso (49), del conocido localmente como Palomo (19), del azul (24), del rojo (4) denominado localmente como “xitocle”, y una población de... |
| Tipo: Tesis |
Palavras-chave: Diversidad genética; Maíz; Chalqueño; Conservación in situ; Isoenzimas; Doctorado; Genética; Genetic diversity; Maize; Chalqueño; In situ conservation; Isozymes. |
| Ano: 2007 |
URL: http://hdl.handle.net/10521/1585 |
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Lázzari,Sonia M. N.. |
| Seventeen clones of Rhopalosiphum padi (Linnaeus, 1758), 14 of R. maidis (Fitch, 1856), and two of R. insertum (Walker, 1849), representing a wide range of host plants and geographic distribution, were examined electrophoretically to determine intra and interspecific variation. Twenty-one enzyme systems were tested using starch-gel techniques. The electromorph variation within species was low, as expected for parthenogenetic organisms. Frequency of heterozygotes was also relatively low for most populations. The percentage of polymorphic loci ranged from 0% to 27.3% in R. padi, but it was lower (0% to 18.2%) in the completely an holocyclic R. maidis. No consistent correlation between band patterns and host plant or geographic origin could be established for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhopalosiphum; Aphididae; Homoptera; Isozymes. |
| Ano: 1992 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751992000100015 |
| |
|
|
| Lozoya-Saldaña,Héctor; Guzmán-Galindo,Leonel; Fernández-Pavia,Sylvia; Grünwald,Niklaus J.; McElhinny,Elaine. |
| Abstract The molecular identity of Phytophthora infestans strains infecting potatoes in the Toluca Valley has been documented. Nevertheless, the relationship of the P. infestans genotypes to the host’s genetic resistance is unclear. In order to identify potential host-pathogen specificity, isolates of the oomycete obtained from five potato varieties with differing levels of disease resistance were compared regarding mating type (MT), isozyme genotype for glucose-6-phosphate isomerase (Gpi) and peptidase (Pep), and sensitivity to metalaxyl. Also, host resistance components and specificity on the host-pathogen interaction were assessed twice in detached leaves in the laboratory and once in attached leaves in the greenhouse for three of the varieties. No... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Compatibility; Isozymes; Metalaxyl resistance; Genetic resistance; Host-pathogen specificity. |
| Ano: 2006 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952006000200205 |
| |
|
| |
|
|
| OLIVEIRA,ALESSANDRO F.; CARVALHO,DULCINÉIA DE; ROSADO,SEBASTIÃO C.S.. |
| Uma população natural de Copaifera langsdorffii Desf., espécie arbórea comumente encontrada no Brasil, foi estudada por meio da eletroforese de isoenzimas, visando determinar a taxa de cruzamento e o sistema reprodutivo. A população amostrada localiza-se ao longo de um trecho do rio Capivari, em uma área de preservação permanente (floresta ciliar), entre os municípios de Lavras e Itumirim, sul do Estado de Minas Gerais, Brasil. Foram amostrados tecidos foliares de 20 indivíduos adultos da população e 400 indivíduos jovens (progênies) procedentes de sementes coletadas de 20 matrizes na população. Cinco sistemas enzimáticos revelaram 24 e 29 alelos totais para os indivíduos adultos e progênies, distribuídos em 12 locos. O sistema reprodutivo desta população... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Copaifera langsdorffii; Isozymes; Hardy-Weinberg Equilibrium. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042002000300009 |
| |
|
| |
|
|
| Orasmo, Gleice Ribeiro; Oliveira-Collet, Sandra Aparecida; Mangolin, Claudete Aparecida; Lapenta, Ana Silvia; Machado, Maria de Fátima Pires da Silva. |
| Current analysis characterizes the effect of different fungicides often applied for pest control on a-and b-esterase patterns of four economically important table-wine grape cultivars (Italia, Rubi, Benitaka and Brasil) of Vitis vinifera. The a- and b-esterase patterns in bud leaves of the cultivars were assessed by native PAGE analysis. Cabrio Top® compound inhibited Est-2, Est-5, Est-6, Est-7, Est-8, Est-9 and Est-10 carboxylesterases, whereas Est-4, Est-11, Est-12, Est-13, Est-14 acetylesterases and Est-16 carboxylesterase were detected as weakly stained bands. Carboxylesterases and acetylesterases were also detected as weakly stained bands when exposed to fungicides Orthocide 500®, Positron Duo® and Folicur PM®. No changes in a- and b-esterase patterns... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.02.00.00-5 esterases; Isozymes; Fungicides; Vine clones; Differential patterns. Genética. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23400 |
| |
| Registros recuperados: 34 | |
|
|
|
